Advances in sedimentation velocity analysis
نویسندگان
چکیده
منابع مشابه
Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.
Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative analysis of macromolecules in solution. AUC has broad applications for the study of biomacromolecules in a wide range of solvents and over a wide range of solute concentrations. Three optical systems are available for the analytical ultracentrifuge (absorbance, interference, and fluorescence) that permi...
متن کاملSedimentation Velocity Analytical Ultracentrifugation
(b) Sedimentation equilibrium: an experiment performed at a lower speed so that the sedimentation and backdiffirsion forces are of comparable magnitudes and therefore give rise to an equilibrium distribution of solute concentration. Because there is no net transport at equilibrium, shape effects do not come into play and the distribution becomes an absolute function of molecular mass for a sing...
متن کاملAnalysis of self-associating systems from sedimentation velocity data.
ANDERSON, P. M., AND MEISTER, A. (1965) Biochemistry 4, 2803-2809 ANDERSON, P. M., AND MEISTER, A. (1966) Biochemistry 6, 3164-3169 PI~RARD, A. (1966) Science 164, 1572-1573 ANDERSON, P. M., AND MARVIN, S. V. (1968) Biochem. Biophys. Res. Commun. 32, 92&934 PIBRARD, A., GLANSDORFF, N., MERGEAY, M., AND WIAME, J. M. (1965) J. Mol. Biol. 14, 23-36 ANDERSON, P. M., AND MEISTER, A. (1966) Biochemis...
متن کامل- ) Methods for Off - line Analysis of Sedimentation Velocity and Sedimentation Equilibrium patterns
The e{uilibrium or transient sedimenration velociry distribution of panicles dispersed in an aqueous solute situated in a cenrifugal accelerative field is routinely studied by means of either an optical trace recorded photographically or by chart recorder traces. Rayleigh intcrferometric fringe patterns have been widely used to record equilibriunt distributions, in which the displacement of the...
متن کاملVelocity correlations and diffusion during sedimentation.
We study the dynamics of sedimenting non-Brownian particles under steady-state conditions in two-dimensional geometry. We concentrate on the autocorrelation functions of the velocity fluctuations and the corresponding memory functions and diffusion coefficients as functions of Phi(V) for small but finite Reynolds numbers. For the numerical simulations we have chosen the model of Schwarzer [Phys...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1997
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(97)78679-x